Formation of Sucrose From Malate in Germinating Castor Beans . II. Reaction Sequence From Phosphoenol-Pyruvate to Sucrose
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Formation of Sucrose From Malate in Germinating Castor Beans . II. Reaction Sequence From Phosphoenol-Pyruvate to Sucrose.
M14late is a key intermiie(liate in converting acetyl units to sucrose in germinating castor beans (8). In an earlier paper evidence wsas presente(d to show that a mechanism exists for the pro(luction of phosphoenol-pyruvate from nmalate in this material andl that phosphoenol-I)yruvate is an interme(liate in the production of sucrose fromii malate (6). In this paper indivi(lual reactions which ...
متن کاملFormation of sucrose from malate in germinating castor beans. I. Conversion of malate to phosphoenol-pyruvate.
composed of all the valence electrons of the specimen. In this case, the electrons of the metal are coupled via the photon gas; their actions are not completely independent. Such a situation is not the case in the usual photosynthetic system; equilibrium or quasiequilibrium ideas may not be applied. In this situation each photosynthetic event is completely independent of the other events taking...
متن کاملPyruvate Dehydrogenase Complex from Germinating Castor Bean
Subceliular organelles from castor bean (Ricuns commnwuis) endosperm were isolated on discontinuous sucrose gradients from germinating seeds which were 1 to 7 days postbit o. Marker enzyme activities of the organeUes were measured (fumarase, catalase, and triose phosphate isomerase) and the homogeneity of the organelle fractions was examined by electron microscopy. Pyrumate dehydrogenase comple...
متن کاملSucrose synthesis from acetate in the germinating castor bean: kinetics and pathway.
During the germination of fatty seeds such as the castor bean, a rapid conversion of fat to sugar occurs (2). The fatty acids are probably broken down to acetyl units via P-oxidation (3) ; the discovery of the glyoxylate cycle enzymes (4) in the castor bean endosperm (5) provided a mechanism whereby these acetyl units could be quantitatively converted to malate. Since malate could be viewed as ...
متن کاملPurification of pyruvate kinase from germinating castor bean endosperm.
Cytosolic pyruvate kinase from endosperm of germinating castor beans (Ricinus communis L.; cv Hale) has been purified 3100-fold to apparent homogeneity and a final specific activity of 203 micromole pyruvate produced/minute per milligram protein. Purification steps included: heat treatment, polyethylene glycol fractionation, Q-Sepharose, ADP-agarose, Mono-Q and Phenyl Superose chromatography. N...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1962
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.37.2.176